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Error in whitelist

See original GitHub issue

Hello, Thank you for such a fantastic tool. I am trying to extract the counts from one of our 10X Genomics datasets.

However, when I run the whitelist command I get the following error

# output generated by whitelist --stdin d1_2_S2_L005_R1_001_short.fastq --bc-pattern=CCCCCCCCCCCCCCCCNNNNNNNNNN --method=umis --log2stderr
# job started at Wed Nov 22 10:47:21 2017 on L-UL-C02QK5VLG8WN.local -- 7d4facbe-56fe-43b5-bbf3-ba91bb0a5ab9
# pid: 3172, system: Darwin 17.2.0 Darwin Kernel Version 17.2.0: Fri Sep 29 18:27:05 PDT 2017; root:xnu-4570.20.62~3/RELEASE_X86_64 x86_64
# blacklist_tsv                           : None
# cell_number                             : False
# compresslevel                           : 6
# error_correct_threshold                 : 1
# expect_cells                            : False
# extract_method                          : string
# filter_cell_barcodes                    : False
# log2stderr                              : True
# loglevel                                : 1
# method                                  : umis
# pattern                                 : CCCCCCCCCCCCCCCCNNNNNNNNNN
# pattern2                                : None
# plot_prefix                             : None
# prime3                                  : None
# random_seed                             : None
# read2_in                                : None
# short_help                              : None
# stderr                                  : <_io.TextIOWrapper name='<stderr>' mode='w' encoding='UTF-8'>
# stdin                                   : <_io.TextIOWrapper name='d1_2_S2_L005_R1_001_short.fastq' mode='r' encoding='UTF-8'>
# stdlog                                  : <_io.TextIOWrapper name='<stderr>' mode='w' encoding='UTF-8'>
# stdout                                  : <_io.TextIOWrapper name='<stdout>' mode='w' encoding='UTF-8'>
# subset_reads                            : 100000000
# timeit_file                             : None
# timeit_header                           : None
# timeit_name                             : all
# whitelist_tsv                           : None
2017-11-22 10:47:21,331 INFO Starting barcode extraction
2017-11-22 10:47:21,385 INFO Parsed 0 reads
2017-11-22 10:47:24,746 INFO Parsed 100000 reads
Traceback (most recent call last):
  File “myHome/anaconda/bin/umi_tools", line 6, in <module>
  File "myHome/anaconda/lib/python3.5/site-packages/umi_tools/", line 59, in main
  File "myHome/anaconda/lib/python3.5/site-packages/umi_tools/", line 310, in main
    barcode_values = ReadExtractor.getBarcodes(read1)
  File "myHome/anaconda/lib/python3.5/site-packages/umi_tools/", line 688, in _getBarcodesString
    umi_quals = [bc_qual1[x] for x in self.umi_bases]
  File "myHome/anaconda/lib/python3.5/site-packages/umi_tools/", line 688, in <listcomp>
    umi_quals = [bc_qual1[x] for x in self.umi_bases]
IndexError: string index out of range

The reads in Read1 are of 26bp long.

Could you please let me know how can I fix it.

Issue Analytics

  • State:closed
  • Created 6 years ago
  • Comments:11 (6 by maintainers)

github_iconTop GitHub Comments

TomSmithCGATcommented, Jan 29, 2018

I think the best solution here is to change the extract method to regex with --extract-method=regex and provide the following regex pattern: --bc-pattern="(?P<cell_1>.{16})(?P<umi_1>.{10})". For any read with less than 26 bp, they will not match the regex but will also not throw an error. The logfile will contain the following lines to describe how many reads matched and how many did not:

INFO Starting barcode extraction
INFO Input Reads: [...]
INFO regex does not match read1: [...]
brianpenghecommented, Jan 18, 2018

Yes. Some reads are too short in our dataset.

Read more comments on GitHub >

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