question-mark
Stuck on an issue?

Lightrun Answers was designed to reduce the constant googling that comes with debugging 3rd party libraries. It collects links to all the places you might be looking at while hunting down a tough bug.

And, if you’re still stuck at the end, we’re happy to hop on a call to see how we can help out.

SyntenyTrack not showing on browser

See original GitHub issue

Describe the bug

SyntenyTrack not showing after following the example in the combined guide to load a synteny track (https://jbrowse.org/jb2/docs/combined/)

To Reproduce

samtools 1.15.1
htslib 1.15.1
@jbrowse/cli/1.6.9 linux-x64 node-v14.18.3 minimap2 2.24-r1122

  1. Run the below commands:
jbrowse create local_jbrowse2 || (rm -rf local_jbrowse2 && jbrowse create local_jbrowse2)
samtools faidx grape.fasta
samtools faidx peach.fasta
minimap2 grape.fasta peach.fasta > peach_vs_grape.paf
jbrowse add-assembly grape.fasta --load copy --out local_jbrowse2 -n grape
jbrowse add-assembly peach.fasta --load copy --out local_jbrowse2 -n peach
jbrowse add-track peach_vs_grape.paf --assemblyNames peach,grape --out local_jbrowse2 --load copy
cd local_jbrowse2 && admin-server
  1. Go to the URL for jbrowse, add the query and target assemblies in the LinearSyntenyView: Screenshot 2022-04-11 at 15 23 23

Please note I am leaving the .paf URL blank as the .paf file is already uploaded in the script above. This leads to the below:

Screenshot 2022-04-11 at 15 22 38

Expected behavior I expect to see the red synteny track as below: linear_synteny-5dde902959edcf331576b5c527ccda51

Version: 1.6.9, linux, installed with conda and running in docker, and accessed with Chrome

Issue Analytics

  • State:closed
  • Created a year ago
  • Comments:5

github_iconTop GitHub Comments

1reaction
Thys3Potgietercommented, Apr 12, 2022

Thanks! Please dont close the ticket yet - I am busy uploading my jbrowse folder to google drive to share - have some difficulties still.

0reactions
cmdcolincommented, Apr 14, 2022

Glad you got it to work! I definitely agree that adding some more comprehensive tutorial steps using the GUI would be very helpful. I wanted to do videos and maybe webpage tutorials here #2448 !

Read more comments on GitHub >

github_iconTop Results From Across the Web

Synteny Tracks - Rat Genome Database
Synteny track popups contain additional information about the syntenic regions in both the “originating” species or assembly (i.e. the species or assembly whose ......
Read more >
Genome Browser User's Guide
To control information overload, tracks need not be displayed in full. Tracks can be hidden, collapsed into a condensed or single-line display, ...
Read more >
Synteny Browser Features
The syntenic block view browser displays the genome features in the reference and comparison species. This consists of five distinct elements (top to...
Read more >
Using the Generic Synteny Browser (GBrowse_syn) - NCBI - NIH
Like GBrowse, GBrowse_syn can be configured to display any organism and is currently the synteny browser used for model organisms such as C....
Read more >
JBrowse 2 guide
Adding a synteny track from a PAF file . ... Figure 2: JBrowse 2 screen showing no configuration found. Click on the sample...
Read more >

github_iconTop Related Medium Post

No results found

github_iconTop Related StackOverflow Question

No results found

github_iconTroubleshoot Live Code

Lightrun enables developers to add logs, metrics and snapshots to live code - no restarts or redeploys required.
Start Free

github_iconTop Related Reddit Thread

No results found

github_iconTop Related Hackernoon Post

No results found

github_iconTop Related Tweet

No results found

github_iconTop Related Dev.to Post

No results found

github_iconTop Related Hashnode Post

No results found