READ TAG: n_elements is zero

Hi,

I am trying to run DeepVariant 1.2.0 on a few human samples PacBio HiFi data (about 30x coverage per sample). I first ran my samples through the PEPPER-Margin pipeline r0.4 to get a haplotagged BAM file. Then I ran DeepVariant as follows:

singularity exec -B ${SOME_PATHS} deepvariant_1.2.0.sif bash /opt/deepvariant/bin/run_deepvariant --model_type PACBIO --ref ${PATH_TO_REF} --reads MARGIN_PHASED.PEPPER_SNP_MARGIN.happlotagged.bam  --output_vcf sample.vcf.gz --output_gvcf sample.g.vcf.gz --num_shards 24 --make_examples_extra_args="realign_reads=false,min_mapping_quality=5" --sample_name MYSAMPLE --use-hp-information;

I have two problems:

1. Right from the beginning (CALL VARIANT MODULE SELECTED), for each interval processed. I get thousands of READ TAG: n_elements is zero messages in the console. What does it mean and is it a problem or just a warning?
2. I allocate 200GB of RAM for per job and they all seem to systematically fail on memory. I do not recall DeepVariant using that much memory in the past but I might be wrong. Is 200GB too light for a human genome PacBio Hifi 30x coverage dataset?

Thank you for your help, Guillaume

[GuillaumeHolley]

Just to confirm: removing the tags worked. Thanks!

[GuillaumeHolley]

Wow, thank you @MariaNattestad @pichuan @williamrowell @kishwarshafin, this all went really quickly. I am currently in the process of generating {fi,fp,ri,rp}-tagless BAM files and will rerun DeepVariant on those. In the meantime, I will close this issue as I am fairly confident you found the solution to the problem. Thank you again!