Error writing output files

Hi,

I tried to run CellBender with the following command: cellbender remove-background \ --input raw_feature_bc_matrix \ --output pool_38-2.h5 \ --expected-cells 5000 \ --total-droplets-included 15000 \ --epochs 300

However, I got the following error in the log file: Encountered an error writing output to file pool_38-2.h5. Output may be incomplete. Encountered an error writing output to file pool_38-2_filtered.h5. Output may be incomplete.

No .h5 output is being produced, only log file, pdf and csv file with cell barcodes, which is not empty.

I have attached log file and output pdf here. pool_38-2.log pool_38-2.pdf

Thanks.

[clemvigh]

I think it may be because I ran CellBender on a server that only had python 3.6.8, and the example with the tiny pbmc dataset gave the same error as above. I tried to run it locally with python 3.7 and that worked fine.

[sjfleming]

Hi @AlexanderAivazidis , from your log file, I cannot tell what has gone wrong unfortunately. I am guessing as you guessed that it may be a pytables issue… but I am not sure.

One thing to try would be to use the docker image, if you don’t want to debug the installation:

us.gcr.io/broad-dsde-methods/cellbender:0.2.0

If you want to test out pytables, I would suggest, in python, trying

import tables
with tables.open_file('some_h5_file.h5') as f:
    print(f)

and make sure that will run